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KMID : 0903520060490030192
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 3 p.192 ~ p.195
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Qualitative PCR Detection of vitamin E-enriched GM Perilla
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Kim Jae-Hwan
Ahn Ji-Hye
Song Hee-Sung
Kim Kyung-Hwan
Kim Dong-Hern
Kim Hae-Yeong
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Abstract
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For the development of a qualitative PCR detection method for genetically modified perilla (Perilla frutescens), perilla species-specific gene, KAS-I (Beta-ketoacyl-ACP synthase I), was selected and validated as suitable for the use as an endogenous reference gene in perilla. Primer specificity was first tested by the means of qualitative PCR analysis. The primer pair Pfru3-F/R amplifying the perilla endogenous gene, KAS-I, gave rise to an amplicon 95 bp. No amplified product was observed when DNA samples from 15 different plants were used as templates. Qualitative PCR detection method was assayed with vitamin E-enriched GM Perilla developed in Korea. For the qualitative PCR detection method, the construct-specific detection primer pairs were constructed. The primer pair TMTO-F/R amplifying the junction region of TMT (γ-tocopherol methyltransferase) gene and OCS (Octopine synthase) terminator introduced in GM perilla gave rise to an amplicon 148 bp.
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KEYWORD
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GM Perilla, γ-tocopherol methyltransferase, β-ketoacyl-ACP synthase I, PCR
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